|
KMID : 0377519880130030367
|
|
Chung-Ang Journal of Medicine
1988 Volume.13 No. 3 p.367 ~ p.380
|
|
|
Histopathologic Studies on the Carcinogen Induced Placental Glutathione S-transferase
|
|
|
|
|
Abstract
|
|
|
A histopathologic and immunohistochemical studies were carried out on the hyperplastic, nodule induced by the Solt and Farber method to observe the morphologic and histochemical enzymatic changes on the hyperplastic hepatocytes with aid of anti-glutathione S-transferase of placenta form (GST-P) antibody.
The results obtained were as follows;
1. The transformed hepatocytes could be detected easily by use of GST-P antibody as early as two weeks later since administration of diethylnitrosoamine only and promoted by additional treatment of acetylaminoflu.orene and partial hepatectomy.
2. The transformed hepatocytes destined to forming hyperplastic nodule were individually scattered at first in multiple foci which is very difficult to find until 3 weeks after posthepatectomy in H &´ E stained section.
3. The transformed hepatocytes consisting of hyperplastic nodule are enlarged clear cells which are proved to have markedly increased amount of glycogen, smooth and rough endoplasmic reticulum by electron microscopy.
4. Area of GST-P positive hepatocytes were progressively and significantly increased by posthepatectomy 3` weeks.
Therefore, glutathione S-transferase is a good marker for. early detection of´ h´yperplastic or preneopalstic nodule induced by diethylnitrosoamine with acetylaminofluorene and the clear hyperplastic hepatocytes was the result of marked increase of glycogen and endoplasmic reticulum.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|